The early use of sepsis scores to predict respiratory failure and mortality in non-ICU patients with COVID-19.

This observational retrospective study aimed to investigate the usefulness of Sequential Organ Failure Assessment (SOFA), Quick SOFA (qSOFA), National Early Warning Score (NEWS), and quick NEWS in predicting respiratory failure and death among patients with COVID-19 hospitalized outside of intensive care units (ICU). We included 237 adults hospitalized with COVID-19 who were followed-up on for one month or until death. Respiratory failure was defined as a PaO2/FiO2 ratio ≤200mmHg or the need for mechanical ventilation. Respiratory failure occurred in 77 patients (32.5%), 29 patients (12%) were admitted to the ICU, and 49 patients (20.7%) died. Discrimination of respiratory failure was slightly higher in NEWS, followed by SOFA. Regarding mortality, SOFA was more accurate than the other scores. In conclusion, sepsis scores are useful for predicting respiratory failure and mortality in COVID-19 patients. A NEWS score ≥4 was found to be the best cutoff point for predicting respiratory failure.

Utilidad de las escalas de sepsis para predecir el fallo respiratorio y la muerte en pacientes con COVID-19 fuera de las Unidades de Cuidados Intensivos / The early use of sepsis scores to predict respiratory failure and mortality in non-ICU patients with COVID-19

El presente estudio retrospectivo observacional tiene como objetivo analizar la utilidad de las escalas SOFA (Sequential Organ Failure Assessment), qSOFA (Quick SOFA), NEWS (National Early Warning Score ) y Quick NEWS para predecir el fallo respiratorio y la muerte en pacientes con COVID-19 atendidos fuera de la Unidad de Cuidados Intensivos (UCI). Se incluyeron 237 adultos con COVID-19 hospitalizados seguidos durante un mes o hasta su fallecimiento. El fallo respiratorio se definió como un cociente PaO2/FiO2 ≤ 200 mmHg o la necesidad de ventilación mecánica. Setenta y siete pacientes (32,5%) desarrollaron fallo ventilatorio; 29 (12%) precisaron ingreso en UCI, y 49 fallecieron (20,7%). La discriminación del fallo ventilatorio fue algo mayor con la puntuación NEWS, seguida de la SOFA. En cuanto a la mortalidad, la puntuación SOFA fue más exacta que las otras escalas. En conclusión, las escalas de sepsis son útiles para predecir el fallo respiratorio y la muerte en COVID-19. Una puntuación ≥ 4 en la escala NEWS sería el mejor punto de corte para predecir fallo respiratorio (AU)

This observational retrospective study aimed to investigate the usefulness of Sequential Organ Failure Assessment (SOFA), Quick SOFA (qSOFA), National Early Warning Score (NEWS), and quick NEWS in predicting respiratory failure and death among patients with COVID-19 hospitalized outside of intensive care units (ICU). We included 237 adults hospitalized with COVID-19 who were followed-up on for one month or until death. Respiratory failure was defined as a PaO2/FiO2 ratio ≤ 200 mmHg or the need for mechanical ventilation. Respiratory failure occurred in 77 patients (32.5%), 29 patients (12%) were admitted to the ICU, and 49 patients (20.7%) died. Discrimination of respiratory failure was slightly higher in NEWS, followed by SOFA. Regarding mortality, SOFA was more accurate than the other scores. In conclusion, sepsis scores are useful for predicting respiratory failure and mortality in COVID-19 patients. A NEWS score ≥ 4 was found to be the best cutoff point for predicting respiratory failure (AU)

[The early use of sepsis scores to predict respiratory failure and mortality in non-ICU patients with COVID-19]. / Utilidad de las escalas de sepsis para predecir el fallo respiratorio y la muerte en pacientes con COVID-19 fuera de las Unidades de Cuidados Intensivos.

This observational retrospective study aimed to investigate the usefulness of Sequential Organ Failure Assessment (SOFA), Quick SOFA (qSOFA), National Early Warning Score (NEWS), and quick NEWS in predicting respiratory failure and death among patients with COVID-19 hospitalized outside of intensive care units (ICU). We included 237 adults hospitalized with COVID-19 who were followed-up on for one month or until death. Respiratory failure was defined as a PaO2/FiO2 ratio ≤ 200 mmHg or the need for mechanical ventilation. Respiratory failure occurred in 77 patients (32.5%), 29 patients (12%) were admitted to the ICU, and 49 patients (20.7%) died. Discrimination of respiratory failure was slightly higher in NEWS, followed by SOFA. Regarding mortality, SOFA was more accurate than the other scores. In conclusion, sepsis scores are useful for predicting respiratory failure and mortality in COVID-19 patients. A NEWS score ≥ 4 was found to be the best cutoff point for predicting respiratory failure.

Effects of feed restriction during pregnancy on maternal reproductive outcome, foetal hepatic IGF gene expression and offspring performance in the rabbit.

Primiparous female rabbits have high nutritional requirements and, while it is recommended that they are subjected to an extensive reproductive rhythm, this could lead to overweight, affecting reproductive outcomes. We hypothesised that restricting food intake during the less energetic period of gestation could improve reproductive outcome without impairing offspring viability. This study compares two groups of primiparous rabbit does in an extensive reproductive programme, one in which feed was restricted from Day 0 to Day 21 of gestation (R021), and another in which does were fed ad libitum (control) throughout pregnancy. The mother and offspring variables compared were (1) mother reproductive outcomes at the time points pre-implantation (Day 3 postartificial insemination [AI]), preterm (Day 28 post-AI) and birth; and (2) the prenatal offspring characteristic IGF system gene expression in foetal liver, liver fibrosis and foetus sex ratio, and postnatal factor viability and growth at birth, and survival and growth until weaning. Feed restriction did not affect the conception rate, embryo survival, or the number of morulae and blastocysts recovered at Day 3 post-AI. Preterm placenta size and efficiency were similar in the two groups. However, both implantation rate (P < 0.001) and the number of foetuses (P = 0.05) were higher in the R021 mothers than controls, while there was no difference in foetal viability. Foetal size and weight, the weights of most organs, organ weight/BW ratios and sex ratio were unaffected by feed restriction; these variables were only affected by uterine position (P < 0.05). Conversely, in the R021 does, foetal liver IGBP1 and IGF2 gene expression were dysregulated despite no liver fibrosis and a normal liver structure. No effects of restricted feed intake were produced on maternal fertility, prolificacy, or offspring birth weight, but control females weaned more kits. Litter weight and mortality rate during the lactation period were also unaffected. In conclusion, pre-implantation events and foetal development were unaffected by feed restriction. While some genes of the foetal hepatic IGF system were dysregulated during pregnancy, liver morphology appeared normal, and the growth of foetuses and kits until weaning was unmodified. This strategy of feed restriction in extensive reproductive rhythms seems to have no significant adverse effects on dam reproductive outcome or offspring growth and viability until weaning.

[Analysis of the characteristics of door-to-door advertising sent by supermarkets in the Principality of Asturias (Spain): a cross-sectional study].

BACKGROUND: To analyze which foods and beverages, frequently included in advertising mail delivered by supermarkets in the Principality of Asturias, can be considered essential for consumption by the population, as well as to compare their price with that of essential products. METHODS: Cross-sectional, descriptive study of supermarket sales circulars delivered to seven homes in Asturias (Spain) by four supermarket chains from July to December 2018. Type of product, its classification as essential or non-essential and mean price of the advertised product were registered. RESULTS: The study identified 14,314 products, mostly belonging to the categories: "cakes, pastries, chocolate, sugar, and sweeteners" (15.3?%), "milk and milk derivatives" (9.7?%), "processed meats".and "alcoholic beverages" (8.9?% each) and "ready-made" (8.4?%). Products considered to be non-essential were found to predominate (61.9?%). Significant differences in product type were observed between supermarket chains. Essential products had a lower mean price than the non-essential products, both overall and for each supermarket chain, and was significantly lower for one of them. . CONCLUSIONS: Products which are non-essential from the point of view of health, such as sugar-rich products, processed meats, and alcoholic beverages, dominated the advertising mail delivered door-to-door by supermarkets in the Principality of Asturias. On a positive note, the prices of essential products, such as fresh fruit and vegetables, were, on average, significantly lower than the prices of non-essential products.

Características de los alimentos y bebidas incluidos en la publicidad buzoneada por supermercados en el Principado de Asturias (España): estudio transversal / Analysis of the characteristics of door-to-door advertising sent by supermarkets in the Principality of Asturias (Spain): a cross-sectional study

FUNDAMENTO: Analizar qué alimentos y bebidas, de los que se incluyen con más frecuencia en la publicidad buzoneada por los supermercados en el Principado de Asturias, pueden ser considerados prescindibles para el consumo de la población, y comparar su precio con el de los alimentos esenciales. MÉTODOS: Estudio descriptivo, transversal, de la publicidad buzoneada en siete domicilios de Asturias (España) por cuatro cadenas de supermercados entre julio y diciembre de 2018. Se registró el tipo de producto, su clasificación como esencial o prescindible y su precio, comparándolos entre cadenas de supermercados. RESULTADOS: Se identificaron 14.314 productos. "Dulces, bollería, chocolates, azúcares y edulcorantes" (15,3 %), "leche y derivados" (9,7 %), "carnes procesadas" y "bebidas alcohólicas" (8,9 % cada categoría) y "precocinados" (8,4 %). Se observó un predominio de productos considerados prescindibles (61,9 %). Se observaron diferencias significativas en cuanto al tipo de producto según la cadena comercial. El precio medio de los productos esenciales fue menor que el de los prescindibles, tanto globalmente como en cada cadena comercial, siendo la diferencia significativa en una de ellas. CONCLUSIONES: En la publicidad buzoneada por los supermercados en los domicilios de Asturias predominan los productos considerados como prescindibles desde el punto de vista de la salud como ricos en azúcar, carnes procesadas y alcohol. En sentido positivo destaca que el precio de los productos esenciales, como frutas y verduras frescas, fue, de media, significativamente menor que el de los prescindibles

BACKGROUND: To analyze which foods and beverages, frequently included in advertising mail delivered by supermarkets in the Principality of Asturias, can be considered essential for consumption by the population, as well as to compare their price with that of essential products. METHODS: Cross-sectional, descriptive study of supermarket sales circulars delivered to seven homes in Asturias (Spain) by four supermarket chains from July to December 2018. Type of product, its classification as essential or non-essential and mean price of the advertised product were registered. RESULTS: The study identified 14,314 products, mostly belonging to the categories: "cakes, pastries, chocolate, sugar, and sweeteners" (15.3 %), "milk and milk derivatives" (9.7 %), "processed meats".and "alcoholic beverages" (8.9 % each) and "ready-made" (8.4 %). Products considered to be non-essential were found to predominate (61.9 %). Significant differences in product type were observed between supermarket chains. Essential products had a lower mean price than the non-essential products, both overall and for each supermarket chain, and was significantly lower for one of them. CONCLUSIONS: Products which are non-essential from the point of view of health, such as sugar-rich products, processed meats, and alcoholic beverages, dominated the advertising mail delivered door-to-door by supermarkets in the Principality of Asturias. On a positive note, the prices of essential products, such as fresh fruit and vegetables, were, on average, significantly lower than the prices of non-essential products

Role of nerve growth factor in the reproductive physiology of female rabbits: A review.

Rabbit does are reflex ovulators such that coitus is needed to release GnRH and elicit the LH surge that triggers the ovulation of mature oocytes. However, the mechanisms eliciting ovulation in this species remain unclear. One of the most promising recently discovered candidates with a role in female reproductive physiology is nerve growth factor beta (ß-NGF). This neurotrophin and its high-affinity receptor TrkA and low affinity receptor p75, is present in all compartments of the ovary, oviduct and uterus suggesting a physiologic role in ovarian folliculogenesis, steroidogenesis, ovulation, luteogenesis and embryo development. Besides, evidence exists that ß-NGF found in seminal plasma could exert a modulatory role in the female hypothalamus-pituitary-ovarian axis contributing to the adrenergic and cholinergic neuronal stimulus of GnRH neurons in an endocrine manner during natural mating. Probably, the paracrine and local roles of the neurotrophin in steroidogenesis and ovulation reinforce the neuroendocrine pathway that leads to ovulation. This review updates knowledge of the role of ß-NGF in rabbit reproduction, including its possible contribution to the mechanisms of action that induce ovulation, and discusses perspectives for the future applications of this neurotrophin on rabbit farms.

Presente y futuro de las nuevas tecnologías en el asma / Present and future of the new technologies in asthma

Los nuevos avances tecnológicos están suponiendo una constante revolución a nivel científico y de relaciones sociales, lo que incluye la relación entre profesionales, la forma de obtención de información y la forma de interacción entre profesionales sanitarios y pacientes. Los profesionales sanitarios estamos obligados a adaptarnos a los cambios tecnológicos de forma crítica para conseguir una utilización racional de los mismos, y en beneficio de los pacientes. En esta revisión analizamos el presente y el futuro de la utilización y aplicación de las nuevas tecnologías en el asma. Analizaremos el uso y los estudios disponibles en telemedicina y aplicaciones móviles diseñadas para pacientes asmáticos, así como la utilización cada vez mayor de dispositivos electrónicos y weareables. Por otro lado, analizaremos la utilidad de las redes sociales dentro de los cuidados de la salud en el asma, su utilización como medio de interacción con los pacientes, y por profesionales sanitarios como método de compartir información y experiencia

The new technological advances are assuming a constant revolution in science and social relations, which includes the relationship between professionals, the way of obtaining information and the form of interaction between health professionals and patients. Health professionals are obligated to adapt to technological changes critically, to achieve a rational use of them, and to use them for the benefit of patients. In this review we analyze the present and future utility and application of new technologies in asthma. In this review we analyze the available studies of telemedicine and mobile applications designed for asthmatic patients, as well as the increasing use of electronic and wearable devices. On the other hand, we analyze the usefulness of social networks within health care in asthma, its use as a method of interaction with patients, and its use by health professionals as a method of sharing scientific information and medical experience

[Coracoclavicular ligaments reconstruction with short lateral peroneal autologous tendon graft]. / Reconstrucción de los ligamentos coracoclaviculares con injerto tendinoso autólogo de peroneo lateral corto.

INTRODUCTION: In the dislocation acromioclavicular unstable vertically, the surgical treatment is elementary by the plasty of the ligaments coracoclaviculares, with the purpose of recovering its stability and normal function. There are techniques of a single tunnel in the clavicle or two tunnels (anatomical), with biological or synthetic material. MATERIAL AND METHODS: It is a longitudinal prospective experimental study; we included 33 patients between 20 and 50 years of age with diagnosis of complete acromioclavicular dislocation with vertical instability. Reconstruction was performed only of the coracoclavicular ligaments with Mazzocas technique of using autologous tendon graft of the short lateral peroneus. The evaluation of results was with the PENN test. RESULTS: 100% of patients are painless and with an arch of normal shoulder mobility one year after surgery. Radiographically, the coracoclavicular distance compared to the healthy side had an average difference of 1.0 mm at rest and 0.9 mm under load. DISCUSSION: The reconstruction of the coracoclavicular ligaments with anatomical technique by autologous tendon graft of the short lateral peroneus, allows to recover the acromioclavicular stability and the normal function of the shoulder.

INTRODUCCIÓN: En la luxación acromioclavicular inestable verticalmente es elemental el tratamiento quirúrgico mediante la reconstrucción de los ligamentos coracoclaviculares, con la finalidad de recuperar su estabilidad y función normal. Existen técnicas de un solo túnel o de dos en la clavícula (anatómica) con material biológico o sintético. MATERIAL Y MÉTODOS: Es un estudio cuasiexperimental prospectivo longitudinal; se incluyeron 33 pacientes entre 20 y 50 años de edad con diagnóstico de luxación acromioclavicular completa con inestabilidad vertical. Se les efectuó plastía sólo de los ligamentos coracoclaviculares con técnica anatómica de Mazzocca usando injerto tendinoso autólogo del peroneo lateral corto. La evaluación de resultados fue con el test de PENN. RESULTADOS: 100% de los pacientes se encuentran sin dolor y con un arco de movilidad normal del hombro un año después de la cirugía. Radiográficamente, la distancia coracoclavicular comparada con el lado sano tuvo una diferencia promedio de 1.0 mm en reposo y 0.9 mm bajo carga. DISCUSIÓN: La plastía tendinosa de los ligamentos coracoclaviculares con técnica anatómica mediante injerto tendinoso autólogo del peroneo lateral corto permite recuperar la estabilidad acromioclavicular y la función normal del hombro a 12 meses de seguimiento.

Reconstrucción de los ligamentos coracoclaviculares con injerto tendinoso autólogo de peroneo lateral corto / Coracoclavicular ligaments reconstruction with short lateral peroneal autologous tendon graft

Resumen: Introducción: En la luxación acromioclavicular inestable verticalmente es elemental el tratamiento quirúrgico mediante la reconstrucción de los ligamentos coracoclaviculares, con la finalidad de recuperar su estabilidad y función normal. Existen técnicas de un solo túnel o de dos en la clavícula (anatómica) con material biológico o sintético. Material y métodos: Es un estudio cuasiexperimental prospectivo longitudinal; se incluyeron 33 pacientes entre 20 y 50 años de edad con diagnóstico de luxación acromioclavicular completa con inestabilidad vertical. Se les efectuó plastía sólo de los ligamentos coracoclaviculares con técnica anatómica de Mazzocca usando injerto tendinoso autólogo del peroneo lateral corto. La evaluación de resultados fue con el test de PENN. Resultados: 100% de los pacientes se encuentran sin dolor y con un arco de movilidad normal del hombro un año después de la cirugía. Radiográficamente, la distancia coracoclavicular comparada con el lado sano tuvo una diferencia promedio de 1.0 mm en reposo y 0.9 mm bajo carga. Discusión: La plastía tendinosa de los ligamentos coracoclaviculares con técnica anatómica mediante injerto tendinoso autólogo del peroneo lateral corto permite recuperar la estabilidad acromioclavicular y la función normal del hombro a 12 meses de seguimiento.

Abstract: Introduction: In the dislocation acromioclavicular unstable vertically, the surgical treatment is elementary by the plasty of the ligaments coracoclaviculares, with the purpose of recovering its stability and normal function. There are techniques of a single tunnel in the clavicle or two tunnels (anatomical), with biological or synthetic material. Material and methods: It is a longitudinal prospective experimental study; we included 33 patients between 20 and 50 years of age with diagnosis of complete acromioclavicular dislocation with vertical instability. Reconstruction was performed only of the coracoclavicular ligaments with Mazzoca's technique of using autologous tendon graft of the short lateral peroneus. The evaluation of results was with the PENN test. Results: 100% of patients are painless and with an arch of normal shoulder mobility one year after surgery. Radiographically, the coracoclavicular distance compared to the healthy side had an average difference of 1.0 mm at rest and 0.9 mm under load. Discussion: The reconstruction of the coracoclavicular ligaments with anatomical technique by autologous tendon graft of the short lateral peroneus, allows to recover the acromioclavicular stability and the normal function of the shoulder.

α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes.

The developmental competence of invitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding α-tocopherol (0, 100, 200 and 400µM) during IVM on putative transcripts involved in antioxidant defence (superoxide dismutase 2, mitochondrial (SOD2), glutathione peroxidase 1 (GPX1), catalase (CAT)), cell cycle regulation and apoptosis cascade (apoptosis tumour protein 53 (TP53), caspase 3, apoptosis-related cysteine protease (CASP3)), cell cycle progression (cellular cycle V-Akt murine thymoma viral oncogene homologue 1 (AKT1)), cumulus expansion (gap junction protein, alpha 1, 43 kDa (GJA1) and prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclo-oxygenase) (PTGS2)) and metabolism (glucose-6-phosphate dehydrogenase (G6PD)). Meiotic progression, mitochondrial reallocation, cumulus cell apoptosis and the developmental competence of oocytes after IVF were also assessed. Expression of SOD2, CAT, TP53, CASP3 and GJA1 was downregulated in cumulus-oocyte complexes (COCs) after IVM with 100µM α-tocopherol compared with the group without the antioxidant. The apoptotic rate and the percentage of a non-migrated mitochondrial pattern were lower in COCs cultured with 100µM α-tocopherol, consistent with better-quality oocytes. In fact, early embryo development was improved when 100µM α-tocopherol was included in the IVM medium, but remained low compared with invivo-matured oocytes. In conclusion, the addition of 100µM α-tocopherol to the maturation medium is a suitable approach to manage oxidative stress and apoptosis, as well as for increasing the in vitro developmental competence of rabbit oocytes.

Riesgo trombótico en varón joven con anomalías cromosómicas / Thrombotic risk in a young male with chromosomal abnormalities

No disponible

Improvements in the conception rate, milk composition and embryo quality of rabbit does after dietary enrichment with n-3 polyunsaturated fatty acids.

This work attempts to confirm the effect of an enriched diet with n-3 polyunsaturated fatty acids (PUFA) trying to mitigate the reproductive performances issues such as low conception rate of primiparous rabbits. A total of 127 does were fed ad libitum throughout their two first cycles with two diets with different fat sources: mixed fat in the control and salmon oil in the enriched one, with 3.19 g/100 g (n=63 does) and 28.77 g/100 g (n=64 does) of n-3 of the total fatty acid, respectively. Feed intake was similar between groups (P>0.05). Plasma progesterone concentration was higher in the enriched females than in control ones at 7 (30.9±2.18 v. 23.9±2.30 ng/ml, respectively; P=0.029) and 14 (38.7±2.18 v. 28.2±2.30 ng/ml, respectively; P=0.001) days of first gestation. Considering both cycles, reproductive parameters of mothers (fertility, duration of gestation and prolificacy) and litter parameters (weight at parturition and weaning, mortality and average daily gain (ADG) of kits during lactation) were similar in both groups. However, individual measurements of neonates of enriched group improved 5.87%, 7.10% and 18.01% (P0.05), but embryo apoptosis rate was higher in control group than in enriched one (31.1±4.56% v. 17.1±3.87%, respectively; P<0.05). In conclusion, dietary PUFA enrichment from the rearing and throughout two productive cycles improved plasma progesterone during pregnancy, fertility, milk fatty acid profile and neonates development of primiparous supporting the beneficial effect of n-3 PUFA supplementation in rabbit does.

A diet supplemented with n-3 polyunsaturated fatty acids influences the metabomscic and endocrine response of rabbit does and their offspring.

The aim of the present study was to evaluate the productive, endocrine, and metabomscic responses as well as oxidative stress of rabbit does and their offspring when fed a diet supplemented with -3 PUFA during their first productive cycle. To this aim, a total of 105 rabbit does were fed ad mscibitum from d 60 to 172 of age 2 isoenergetic and isoproteic diets differing in fatty acid composition. The control diet ( = 52 does) contained 45.9 g/kg of -3 of the total fatty acids and the enriched diet ( = 53 does) contained 149.2 g/kg of -3 of the total fatty acids. Both experimental groups had similar feed intake during rearing, pregnancy, and lactation. The enrichment of diet had no effect on ultrasonographic assessment of does on d 9 and 16 of pregnancy, with an embryonic vesicle number and fetus and placenta size similar between groups ( > 0.05). Even though there were no major effects ( > 0.05) on fertimscity, duration of gestation, and number born amscive and stillborn kits at parturition, mscive kits from enriched does were longer (71.6 ± 2.42 vs. 79.5 ± 2.13 mm; < 0.05) and tended to be heavier (42.5 ± 3.94 vs. 50.8 ± 3.47 g; = 0.07) than those from control does ( < 0.05). The 2 groups had similar milk production and mortamscity values during lactation; consequently, there were no differences between diets in ADG, mscitter weight, and number of weaned kits ( > 0.05). In enriched does, higher plasma leptin and estradiol concentrations than in control does ( < 0.05) were observed. In addition, enriched females also had lower total and high-density mscipoprotein cholesterol (HDL-c) than control females during lactation ( < 0.05). Regarding offspring, the enrichment of diet with PUFA caused a hypermscipidemic status (greater values of plasma triglycerides, total cholesterol, and HDL-c; < 0.05) at 1 d postpartum (dpp), compared with the control group, that disappeared at 32 dpp. Supplemented does before parturition and their offspring at 1 dpp had greater oxidative stress than those in the control group. In conclusion, an increase of -3 PUFA concentration in the diet of rabbit does and, consequently, of their offspring during a productive cycle alters their mscipid profile and the indicators of oxidative stress, without major endocrine modifications or improvements in the productive variables.

In vivo and in vitro maturation of rabbit oocytes differently affects the gene expression profile, mitochondrial distribution, apoptosis and early embryo development.

In vivo-matured cumulus-oocyte complexes are valuable models in which to assess potential biomarkers of rabbit oocyte quality that contribute to enhanced IVM systems. In the present study we compared some gene markers of oocytes and cumulus cells (CCs) from immature, in vivo-matured and IVM oocytes. Moreover, apoptosis in CCs, nuclear maturation, mitochondrial reallocation and the developmental potential of oocytes after IVF were assessed. In relation to cumulus expansion, gene expression of gap junction protein, alpha 1, 43 kDa (Gja1) and prostaglandin-endoperoxide synthase 2 (Ptgs2) was significantly lower in CCs after in vivo maturation than IVM. In addition, there were differences in gene expression after in vivo maturation versus IVM in both oocytes and CCs for genes related to cell cycle regulation and apoptosis (V-Akt murine thymoma viral oncogene homologue 1 (Akt1), tumour protein 53 (Tp53), caspase 3, apoptosis-related cysteine protease (Casp3)), oxidative response (superoxide dismutase 2, mitochondrial (Sod2)) and metabolism (glucose-6-phosphate dehydrogenase (G6pd), glyceraldehyde-3-phosphate dehydrogenase (Gapdh)). In vivo-matured CCs had a lower apoptosis rate than IVM and immature CCs. Meiotic progression, mitochondrial migration to the periphery and developmental competence were higher for in vivo-matured than IVM oocytes. In conclusion, differences in oocyte developmental capacity after IVM or in vivo maturation are accompanied by significant changes in transcript abundance in oocytes and their surrounding CCs, meiotic rate, mitochondrial distribution and apoptotic index. Some of the genes investigated, such as Gja1, could be potential biomarkers for oocyte developmental competence in the rabbit model, helping improve in vitro culture systems in these species.

Cáncer de pulmón en la provincia de Ávila. Tasas de incidencia, epidemiología del año 2012 y tendencias en los últimos 20 años / Lung cancer in Avila province, Spain. Incidence rates, epidemiolgy of the year 2012 and trends in the last 20 years

Antecedentes y objetivos. Nos proponemos conocer la magnitud del cáncer de pulmón en Ávila: sus tasas de incidencia y aspectos epidemiológicos importantes en el año 2012, comparándolo con los resultados de estudios conocidos realizados con la misma metodología cada 5 años, desde hace ya 2 décadas. Pacientes y métodos. Inclusión prospectiva de todos los pacientes diagnosticados de cáncer de pulmón en la provincia de Ávila a lo largo del año 2012. Resultados. Se diagnosticaron 81 enfermos, 70 hombres y 11 mujeres, con edad media de 72,1 años (extremos: 44-91), superior a la encontrada en estudios anteriores. Dan lugar a unas tasas de incidencia en 2012, brutas y ajustadas a la población mundial estándar, de 80,99 y 31,23 por 100.000, respectivamente, en los hombres, y 12,97 y 5,68 por 100.000 en las mujeres. Estas tasas son menores, en ambos sexos, a las encontradas en Ávila en el año 2002. En 2012 habían sido fumadores el 80,25% (90% de los hombres y 18,18% de las mujeres), aunque al diagnóstico lo habían dejado el 68,75%. Realizamos diagnóstico clínico-radiológico en 9 (11,1%) y cito-histológico en 72 (88,9%). Tipos histológicos: adenocarcinomas 37,5%; escamosos 33,3%; microcíticos 13,8%; indiferenciados no microcíticos 11,1%; células grandes 2,77% y carcinoides 1,38%. Los tratamientos más frecuentes fueron quimioterapia (50,6%), sintomático (23,4%) y cirugía (12,3%). Conclusiones. En los últimos 10 años disminuye la incidencia del cáncer de pulmón en Ávila en ambos sexos. En 2012 los pacientes han sido más ancianos, predominando la histología adenocarcinoma y el tratamiento con quimioterapia (AU)

Background and objectives. To determine the extent of lung cancer in Alvila. Its incidence rates and significant epidemiological aspects of the year 2012 were recorded, and the results of each 5-year period (up to 20 years) were compared with those of known studies conducted using the same methodology. Patients and methods. A prospective study was conducted on all patients diagnosed with lung cancer in the Province of Avila throughout the year 2012. Results. A total of 81 patients were diagnosed, of whom 70 were males and 11 females, with a mean age of 72.1 years (range: 44-91), and was higher than that found in previous studies. This gave gross, and adjusted to the standard world population, incidence rates in 2012 of 80.99 and 31.23 per 100,000, respectively, in males, and 12.97 and 5.68 per 100,000, respectively in females. These rates are lower in both sexes than those found in Alvila in 2002. In 2012, 80.25% had been smokers (90% of males and 18.18% of the women), although, on diagnosis, 68.75% had quit smoking. A clinical-radiological diagnosis was made in 9 (11.1%), with a histocytological diagnosis in 72 (88.9%). The histological types were: adenocarcinomas in 37.5%; squamous in 33.3%; microcytic in 13.8%; undifferentiated non-small cell in 11.1%; large cell in 2.77%, and carcinoid in 1.38%. The most frequent treatments were chemotherapy (50.6%), symptomatic (23.4%), and surgery (12.3%). Conclusions. The incidence of lung cancer in Avila has decreased in both sexes in the last 10 years. In 2012, the patients have been older, the majority with adenocarcinoma histology, and receiving chemotherapy (AU)

Bronquiectasias y asma / No disponible

No disponible